A comprehensive review on microbial l ‐asparaginase: Bioprocessing, characterization, and industrial applications

Graphical summary ofl‐asparaginase. Abstractl‐Asparaginase (E.C.3.5.1.1.) is a vital enzyme that hydrolyzesl‐asparagine tol‐aspartic acid and ammonia. This property ofl‐asparaginase inhibits the protein synthesis in cancer cells, makingl‐asparaginase a mainstay of pediatric chemotherapy practices to treat acute lymphoblastic leukemia (ALL) patients.l‐Asparaginase is also recognized as one of the important food processing agent. The removal of asparagine byl‐asparaginase leads to the reduction of acrylamide formation in fried food items.l‐Asparaginase is produced by various organisms including animals, plants, and microorganisms, however, only microorganisms that produce a substantial amount of this enzyme are of commercial significance. The commerciall‐asparaginase for healthcare applications is chiefly derived fromEscherichia coli andErwinia chrysanthemi. A high rate of hypersensitivity and adverse reactions limits the long ‐term clinical use ofl‐asparaginase. Present review provides thorough information on microbiall‐asparaginase bioprocess optimization including submerged fermentation and solid‐state fermentation forl‐asparaginase production, downstream purification, its characterization, and issues related to the clinical application including toxicity and hypersensitivity. Here, we have highlighted the bioprocess techniques that can produce improved and economically viable yields ofl‐asparaginase from promising microbial sources in the cur...
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: Review Article Source Type: research