Whole genome sequence analysis of cell culture-adapted rotavirus A strains from chicken.

Whole genome sequence analysis of cell culture-adapted rotavirus A strains from chicken. Infect Genet Evol. 2020 Mar 05;:104275 Authors: Patzina-Mehling C, Falkenhagen A, Gadicherla AK, Grützke J, Tausch SH, Johne R Abstract Rotavirus A (RVA) is a major cause of gastroenteritis in humans and mammalian animals, and has also been abundantly detected in avian species. Avian RVA infection is associated with diarrhea, reduced growth and increased mortality, leading to economic losses in the poultry industry. Avian RVA forms a unique genetic clade within the whole RVA species. However, up to now, only a few full-length avian RVA genomes have been published and only a small number of avian RVA strains have been adapted to grow in cell culture for subsequent studies. Here, the four cell culture-adapted chicken RVA strains 02V0002G3, 04V0027G6, 05V0500F6 and 06V0661G1 were characterized in more detail. Transmission electron microscopy of the viruses derived from culture supernatant showed a typical triple-layered morphology of rotavirus particles; in addition, strain 06V0661G1 showed a high proportion of double-layered particles. The (nearly) complete genome sequences of the viruses were determined using next-generation sequencing (NGS). The resulting sequences were compared to full-length or partial sequences of the strains previously determined using Sanger sequencing; and a few nucleotide mismatches, some of them resulting in amino acid substitutions, wer...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research

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Authors: Noda A, Sakai T, Obara T, Miyazaki M, Tsuchiya M, Oyanagi G, Murai Y, Mano N Abstract BACKGROUND: There are no reports on investigations of the characteristics of adverse drug reaction (ADR) reports for pediatric patients in the Japanese Adverse Drug Event Report database (JADER) and the utility of database for drug safety surveillance in these patients. METHOD: We aimed to evaluate ADR reports for pediatric patients in the JADER. We used spontaneous ADR reports included in the JADER since April 1, 2004, to December 31, 2017, which was downloaded in April 2018. In a total of 504,407 ADR reports, the nu...
Source: BMC Pharmacology and Toxicology - Category: Drugs & Pharmacology Tags: BMC Pharmacol Toxicol Source Type: research
In the May 2020 issue of BioScience, AIBS President Dr. Charles Fenster and Executive Director Dr. Robert Gropp urge governments to coordinate to promote greater understanding of the SARS-CoV-2 pandemic, including the development of a vaccine. They further call on governments to “build the infrastructure needed to foster timely scientific exchange and the conversion of scientific information into responsible public policy - both domestically and internationally, for the current and future crises.” They further warn, “Understanding the origins of the current global health crisis resulting from the novel c...
Source: Public Policy Reports - Category: Biology Authors: Source Type: news
Vaccination is a mainstay in preventive medicine, reducing morbidity and mortality from infection, largely by generating pathogen-specific neutralizing antibodies. However, standard immunization strategies are insufficient with increasing age due to immunological impediments, including defects in T follicular helper (Tfh) cells. Here, we found that Tfh generation is inversely linked to the expression of the ecto-NTPDase CD39 that modifies purinergic signaling. The lineage-determining transcription factor BCL6 inhibited CD39 expression, while increased Tfh frequencies were found in individuals with a germline polymorphism p...
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
Publication date: 5 November 2020Source: Journal of Hazardous Materials, Volume 398Author(s): Huan He, Shijie Sun, Jiong Gao, Bin Huang, Tianguo Zhao, Hongyu Deng, Xiaoxia Wang, Xuejun Pan
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
The firm will develop viral vector vaccines from IAVI and Themis Bioscience, and the experimental EIDD-2801 antiviral compound developed by Ridgeback Biotherapeutics
Source: Chemical and Engineering News - Category: Chemistry Authors: Source Type: research
CONCLUSION: Our results indicate that Fc molecule facilitate immune responses and PEDV harbouring Fc molecule could be a possible vaccine candidate. However, a challenge experiment would be needed to investigate the protective efficacy of PEDV harbouring Fc. PMID: 32448096 [PubMed - as supplied by publisher]
Source: Veterinary Quarterly - Category: Veterinary Research Tags: Vet Q Source Type: research
Source: BMJ News - Category: General Medicine Source Type: research
Publication date: Available online 25 May 2020Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): B.G.S.S. Gamage, D.R.A. Dissanayake, I.D. Silva, D.V.P. Prasada
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
This study aimed to determine the centesimal composition and physicochemical characteristics of the pulp and seed of S. cumini (L.) Skeels, as well as the content of total phenolic compounds and the antioxidant, antibacterial, antibiofilm and anti-quorum sensing (QS) activities of the phenolic extracts obtained from the pulp and the seeds of this fruit. The in vitro antibacterial and anti-QS activities of active films incorporating phenolic extracts were also evaluated. Additionally, we performed molecular docking of phenolic compounds present in jambolan with the CviR QS regulator of Chromobacterium violaceum. The composi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, antibiotic-resistant strains from marine sediment (n = 50) and clams (n = 53) were analyzed (i) for their plasmid content using a PCR-based plasmid replicon typing (PBRT) kit and (ii) for the transferability of plasmid-associated antibiotic resistance (AR) traits by mating experiments. Fifteen of the thirty replicons targeted by the PBRT kit were detected in the isolates; 8/15 were identified in both sediment and clam isolates, although at different frequencies. The most frequent replicons in sediment (74%) and in clam strains (66%) alike, were FIA, FIB, or FII, which are associated with the IncF group, foll...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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