Anti ‐invasive effect and pharmacological mechanism of genistein against colorectal cancer

AbstractColorectal cancer (CRC) refers to a deadly carcinoma following potent invasiveness and metastasis in advanced stage. Unfortunately, existing anti ‐CRC medicine is insufficient for chemotherapy in addition to adverse effects. Consequently, the candidate natural ingredient for treating CRC needs to be further developed. Our previous experiments report that genistein exerts beneficial effects to inhibit CRC cells via an antiproliferative mecha nism. Based on the metastatic characteristics of staging CRC, anti‐invasive and antimetastatic pharmacological activities using genistein remain uninvestigated. The scientific purpose of this study was to disclose the antimetastatic mechanism by using human and cell culture/nude mice samples, foll owed by biochemical tests and immunoassays. In human study, these CRC cases resulted in increased transforming growth factor beta‐1 (TGF‐β1) levels, long noncoding RNA (lncRNA) TTTY18 expressions, followed with up‐regulated Ki‐67, serum and glucocorticoid regulated kinase 1 (SGK1), AktSer473 expressions. In a study in vitro, genistein ‐dosed CRC cells showed suppressed cell viability, promoted cell apoptosis, reduced Ki‐67 positive cells, reduced cellular migration, down‐regulated expressions of TTTY18, SGK1, AktSer473, p38 MAPKTyr323. In a further study in vivo, genistein ‐dosed tumor‐bearing nude mice exhibited visibly reduced body mass, lowered tumorous TGF‐β1 and TTTY18 contents. In addition, intracellular nu...
Source: BioFactors - Category: Biochemistry Authors: Tags: Research Communication Source Type: research