Screening for scFv-fragments that are stable and active in the cytosol.

In this study, we evaluated fast and simple screening methods to identify scFv fragments that are stable and functional in the cytosol. We analyzed various phage display derived human scFv antibodies recognizing extracellular signal-regulated kinase 2 (Erk2) for stability and antigen binding under reducing and non-reducing conditions. Further, we developed an assay allowing to measure the interaction of the scFv intrabodies with their antigen in the cytosol of in living cells, by using a Split-Luciferase (Split-Luc) assay. ScFv fragments showing antigen binding in the cytosol could successfully be identified. PMID: 32116242 [PubMed - as supplied by publisher]
Source: Human Antibodies - Category: Biochemistry Tags: Hum Antibodies Source Type: research