Down ‐regulation of miR‐383‐5p suppresses apoptosis in oxidative stress rat hepatocytes by targeting Bcl2

AbstractmiRNAs are a class of small non ‐coding RNAs that are involved in various biological processes. In the preliminary work of the laboratory, found that miR‐383‐5p was down‐regulated in the liver tissue of acute cold stress rats and has been shown to be an important regulatory factor in tumour proliferation, but there are ver y few studies involving the mediation of cold stress in rat liver tissues. Therefore, the purpose of this study was to determine the effect of miR‐383‐5p on the livers of cold stress rats by simulating the cold stress state of rat liver tissues in vitro using H2O2 to induce rat hepatocyte oxidative stress. The results showed that MDA content, Caspase 3 and Cyto C protein levels increased significantly; GPx activity and SOD1 protein levels decreased significantly and miR ‐383‐5p expression was significantly down‐regulated in rat liver tissues after cold stress. Different concentrations of H2O2 was added to rat hepatocytes, and the results showed that the expression of miR ‐383‐5p, the ROS level, and the apoptosis rate in rat hepatocytes was increased significantly in a concentration‐dependent fashion. Transfection of miR‐383‐5p inhibitor revealed that the apoptosis rate of rat hepatocytes, and the protein level of apoptosis‐related protein Caspase 3 were reduced; the results of the dual‐luciferase reporter gene assay showed that miR‐383‐5p targeted regulation of Bcl2. The results suggested that the expression of mi...
Source: Journal of Animal Physiology and Animal Nutrition - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research