Rapid differential detection of Genotype I and III Japanese Encephalitis Virus from clinical samples by a novel duplex TaqMan probe-based RT-qPCR assay.

This study aimed to establish a rapid JEV genotyping method using novel duplex TaqMan RT-qPCR assay.specific primer and probes located in the PrM/M gene that were able to specifically differentiate GI and GIII JEV, was selected as the duplex TaqMan RT-qPCR target.The specificity, sensitivity and reproducibility test of this assay were validated. The sensitivity of the assay was 10 genomic RNA copies for both GI and GIII JEV in field mosquito and pig samples,and more sensitive than the current methods. In addition, the novel assay can be completed in less than 1 h. Therefore, This duplex TaqMan RT-qPCR assay is a promising tool for rapid differential detection and epidemiology of GI and GIII JEV strains in China. The results showed that co-circulation of GI and GIII infections with GI infection being more prevalent in pigs or mosquito in eastern China.
PMID: 32105753 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - February 23, 2020 Category: Virology Authors: Wang X, Guo S, Hameed M, Zhang J, Pang L, Li B, Qiu Y, Liu K, Shao D, Ma Z, Zhong D, Wei J, Li P Tags: J Virol Methods Source Type: research