Screening and Quantification of TNF- α Ligand from Angelicae Pubescentis Radix by Biosensor and UPLC-MS/MS.

Screening and Quantification of TNF-α Ligand from Angelicae Pubescentis Radix by Biosensor and UPLC-MS/MS. Anal Biochem. 2020 Feb 25;:113643 Authors: Yang L, Hou A, Wang S, Zhang J, Man W, Guo X, Yang B, Wang Q, Jiang H, Kuang H Abstract The aim of this study is to establish a method for rapid screening of active ingredients targeting TNF-α from Chinese herbal medicines. Take Angelicae Pubescentis Radix (APR) as an example, surface plasma resonance technique was used to establish for screening small molecule inhibitors of TNF-α from APR extract. Then UPLC-MS/MS coupled with chemometric was used for quantitative and evaluate the differences of the candidate compounds bound to TNF-α in APR from different sources. In the experiment, TNF-α protein was fixed on the CM5 chip surface of biacore T200 biosensor by amino coupling. A series of small molecular compounds in APR were screened and six phenolic acid compounds had a strong affinity for TNF-α protein and could be used as TNF-α antagonists. In summary, the targeted drug screening method for TNF-α protein based on SPR technology established in this study can be used to screen anti-TNF-α small molecule inhibitors. UPLC-MS/MS can accurately quantify 15 active ingredients, which provides reliable experimental data and new research ideas for targeted drug research on TNF-α protein. PMID: 32105738 [PubMed - as supplied by publisher]
Source: Analytical Biochemistry - Category: Biochemistry Authors: Tags: Anal Biochem Source Type: research