Plant organellar DNA polymerases bypass Thymine Glycol using two conserved lysine residues.

Plant organellar DNA polymerases bypass Thymine Glycol using two conserved lysine residues. Biochem J. 2020 Feb 28;: Authors: Baruch-Torres N, Yamamoto J, Juárez-Quintero V, Iwai S, Brieba LG Abstract Plant organelles cope with endogenous DNA damaging agents, byproducts of respiration and photosynthesis, and exogenous agents like ultraviolet light. Plant organellar DNA polymerases (DNAPs) are not phylogenetically related to yeast and metazoan DNAPs and they harbor three insertions not present in any other DNAPs. Plant organellar DNAPs from Arabidopsis thaliana (AtPolIA and AtPolIB) are translesion synthesis (TLS) DNAPs able to bypass abasic sites, a lesion that poses a strong block to replicative polymerases. Besides abasic sites, reactive oxidative species and ionizing radiation react with thymine resulting in thymine glycol (Tg), a DNA adduct that is also a strong block to replication. Here we report that AtPolIA and AtPolIB bypass Tg by inserting an adenine opposite the lesion and efficiently extend from a Tg-A base pair. The TLS ability of AtPolIB is mapped to two conserved lysine residues: K593 and K866. Residue K593 is situated in insertion 1 and K866 is in insertion 3.  With basis on the location of both insertions on a structural model of AtPolIIB we hypothesize that the two positively charged residues interact to form a clamp around the primer-template. In contrast to nuclear and bacterial replication, where lesion bypass ...
Source: The Biochemical Journal - Category: Biochemistry Authors: Tags: Biochem J Source Type: research