Discovery of novel molecular characteristics and cellular biological properties in ameloblastoma

In this study, we sought to uncover the molecular basis of ameloblastoma and to determine the cellular phenotype of ameloblastoma cells withBRAF mutations. Our comparative cDNA microarray analysis and gene set enrichment analysis (GSEA) showed that ameloblastoma exhibited a distinct gene expression pattern from the normal tissues: KRAS ‐responsive gene set is significantly activated in ameloblastoma. Importantly,insulin like growth factor 2 (IGF2), a member of KRAS ‐responsive genes, enhances the proliferation of an ameloblastoma cell line AMU‐AM1 withBRAF mutation. In addition, Toll ‐like receptor 2 (TLR2) knockdown readily inactivated KRAS‐responsive gene sets as well as increases caspase activities, suggesting that TLR2 signaling may mediate cell survival signaling in ameloblastoma cells. Collectively, the findings may help to further clarify the pathophysiology of amelob lastoma and lead to the development of precision medicine for patients with ameloblastoma.
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research