Analysis of protein missense alterations by combining sequence ‐ and structure‐based methods

ConclusionCombining different computational approaches including some that were not developed to investigate missense variants help to predict the possible impact of the amino acid substitutions. Yet, when the modified residues are involved in a salt ‐bridge, the tools tend to fail, even when the analysis is performed in 3D. Thus, interactive structural analysis with molecular graphics packages such as Chimera or PyMol or others are still needed to clarify automatic prediction.

Source: Molecular Genetics & Genomic Medicine - February 27, 2020 Category: Genetics & Stem Cells Authors: Aram Gyulkhandanyan, Alireza R. Rezaie, Lubka Roumenina, Nathalie Lagarde, Veronique Fremeaux ‐Bacchi, Maria A. Miteva, Bruno O. Villoutreix Tags: ORIGINAL ARTICLE Source Type: research