Role of Epoxyeicosatrienoic Acids (EETs) in Mediation of Dopamine's Effects in the Kidney.

Role of Epoxyeicosatrienoic Acids (EETs) in Mediation of Dopamine's Effects in the Kidney. Am J Physiol Renal Physiol. 2013 Oct 23; Authors: Zhang MZ, Wang Y, Yao B, Gewin L, Wei S, Capdevila JH, Harris RC Abstract We have recently demonstrated that intrarenal dopamine plays an important role in preventing the development of systemic hypertension. Similarly, renal cytochrome P450 (CYP)-epoxygenase-derived arachidonic acid metabolites, epoxyeicosatrienoic acids (EETs) also are anti-hypertensive through inhibiting sodium reabsorption and vasodilation. The potential interaction between renal dopamine and epoxygenase systems was investigated. COMT(-/-) mice with increased intrarenal dopamine levels and ptAADC(-/-) with renal dopamine deficiency were treated with low-salt diet or high-salt diet for 2 weeks. Wild type or Cyp2c44(-/-) mice were treated with gludopa, which selectively increased renal dopamine levels. In low-salt treated mice, urinary EET levels were related to renal dopamine levels, being highest in COMT(-/-) mice and lowest in ptAADC(-/-) mice. In high-salt treated mice, total EET and individual EET levels in both kidney and urine were also highest in COMT(-/-) mice and lowest in ptAADC(-/-) mice. Selective increases in renal dopamine in response to gludopa administration led to marked increases in both total and all individual EET levels in the kidney without any changes in blood levels. qRT-PCR and Western analysis indicated that g...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research