Genomic epidemiology of complex, multi-species, plasmid-borne bla KPC carbapenemase in Enterobacterales in the UK, 2009-2014.

Genomic epidemiology of complex, multi-species, plasmid-borne bla KPC carbapenemase in Enterobacterales in the UK, 2009-2014. Antimicrob Agents Chemother. 2020 Feb 24;: Authors: Stoesser N, Phan HTT, Seale AC, Aiken Z, Thomas S, Smith M, Wyllie D, George R, Sebra R, Mathers AJ, Vaughan A, Peto TEA, Ellington MJ, Hopkins KL, Crook DW, Orlek A, Welfare W, Cawthorne J, Lenney C, Dodgson A, Woodford N, Walker AS, TRACE Investigators' Group Abstract Carbapenem resistance in Enterobacterales is a public health threat. Klebsiella pneumoniae carbapenemase (encoded by alleles of the bla KPC family) is one of the commonest transmissible carbapenem resistance mechanisms worldwide. The dissemination of bla KPC has historically been associated with distinct K. pneumoniae lineages (clonal group 258 [lsqb]CG258[rsqb]), a particular plasmid family (pKpQIL), and a composite transposon (Tn4401). In the UK, bla KPC has represented a large-scale, persistent, management challenge for some hospitals, particularly in North-West England. The dissemination of bla KPC has evolved to be polyclonal and poly-species, but the genetic mechanisms underpinning this evolution have not been elucidated in detail; this study used short-read whole genome sequencing of 604 bla KPC-positive isolates (Illumina) and long-read assembly (PacBio)/polishing (Illumina) of 21 isolates for characterisation. We observed the dissemination of bla KPC (predominantly bla KPC-2; 573/604 ...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research