Isoforms of Cathepsin B1 in Neurotropic Schistosomula of Trichobilharzia regenti Differ in Substrate Preferences and a Highly Expressed Catalytically Inactive Paralog Binds Cystatin

Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate specificity and after processing it was less effective toward protein substrates when compared to TrCB1.1. Self-processing of both isoforms could be facilitated by sulfated polysaccharides due to a specific binding motif in the pro-sequence. Trans-activation by heterologous enzymes was also successfully employed. Expression profiling revealed a high level of transcription of genes encoding the enzymatically inactive paralogs TrCB1.5 and TrCB1.6. The transcription level of TrCB1.6 was comparable with that of TrCB1.1 and TrCB1.2, the most abundant active isoforms. Recombinant TrCB1.6wt, a wild type paralog with a Cys29-to-Gly substitution in the active site that renders the enzyme inactive, was processed by the active TrCB1 forms and by an asparaginyl endopeptidase. Although TrCB1.6wt lacked hydrolytic activity, endopeptidase, but not dipeptidase, activity could be restored by mutating Gly29 to Cys29. The lack of exopeptidase activity may be due to other mutations, such as His110-to-Asn in the occluding loop and Asp224-to-Gly in the main body of the mature TrCB1.6, which do not occur in the active isoforms TrCB1.1 and TrCB1.4 with exopeptidase activity. The catalyti...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Conclusion: CoBaT-Y017, a Phase II study was conducted with Plasmodium falciparum malaria-infected patients, for efficacy and safety evaluation of CoBaT-Y017 compared with Artemether-Lumefantrine used as a positive control. Methods. A single-blind randomized trial was conducted on 25 eligible males aged 18-40 years randomly assigned to two treatment groups: CoBaT-Y017 or Artemether-Lumefantrine. The first group received 35 ml of CoBaT-Y017 in 1.5 L mineral water administered daily for four consecutive days; the second group received oral Artemether-Lumefantrine, using WHO-recommended therapeutic dose regimens...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Publication date: Available online 28 March 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Roberta Iatta, Alda Natale, Silvia Ravagnan, Jairo Mendoza-Roldan, Andrea Zatelli, Maria Alfonsa Cavalera, Yaarit Nachum-Biala, Gad Baneth, Domenico Otranto
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
CrystEngComm, 2020, Advance Article DOI: 10.1039/C9CE01971E, PaperDaisuke Nakamura, Taishi Kimura, Kenji Itoh, Naoki Fujimoto, Shugo Nitta, Hiroshi Amano Catalytic tungsten carbide coatings provide multi-functional (w/ anti-parasitic-reaction and protective functions) reactor components for growth of large GaN crystals. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - CrystEngComm latest articles - Category: Chemistry Authors: Source Type: research
(Natural News) Parasites can be a massive headache in a microscopic size, and in the case of a certain parasitic worm, the headache it causes is literal – the rat lungworm parasite can wreak havoc on a person’s brain, and can even cause major neurological problems. Now, recent findings by the Hawaii State Department of...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Herring worms can be found in a variety of raw fish and squid More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=300267&WT.mc_id=USNSF_1 This is a Research News item.
Source: NSF Discoveries - Category: Science Source Type: research
Imperatore Malaria is a life-threatening disease and, what is more, the resistance to available antimalarial drugs is a recurring problem. The resistance of Plasmodium falciparum malaria parasites to previous generations of medicines has undermined malaria control efforts and reversed gains in child survival. This paper describes a continuation of our ongoing efforts to investigate the effects against Plasmodium falciparum strains and human microvascular endothelial cells (HMEC-1) of a series of methoxy p-benzyl-substituted thiazinoquinones designed starting from a pointed antimalarial lead candidate. The data obta...
Source: Molecules - Category: Chemistry Authors: Tags: Communication Source Type: research
This study investigates the presence of this parasite in 296 cats from Murcia municipality (SE Spain), and describes possible factors associated with the infestation. Cats were grouped by sex, age, lifestyle, season and provenience. Scraping samples were examined by a microscope to identify the mite. Chi square test was computed and odds ratio was used to measure the association of risk factors with parasite prevalence. Additionally, the spatial distribution of prevalences was investigated and represented through GIS software. Around 30% of the cats (CI95 25 –35%) were found positives toO. cynotis. The mite infestati...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
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Source: Paediatrics and international child health - Category: Pediatrics Tags: Paediatr Int Child Health Source Type: research
Publication date: Available online 25 March 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Ian Beveridge
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Source: Mitochondrion - Category: Biochemistry Source Type: research
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