Directive effect of chain length in modulating peptide nano-assemblies.

Directive effect of chain length in modulating peptide nano-assemblies.
Protein Pept Lett. 2020 Feb 23;:
Authors: Pandey G, Das PP, Ramakrishnan V
Abstract
RADA-4 (Ac-RADARADARADARADA-NH2) is the most extensively studied and marketed self- assembling peptide, forming hydrogel, used to create defined three-dimensional microenvironments for cell culture applications. In this work, we use various biophysical techniques to investigate the length dependency of RADA aggregation and assembly. We synthesized a series of RADA-N peptides, N ranging from 1 to 4, resulting in four peptides having 4, 8, 12, and 16 amino acids in their sequence. Our observations show that the aggregation of ionic, charge-complementary RADA motif-containing peptides is length-dependent, with N less than 3 are not forming spontaneous self-assemblies.
PMID: 32091324 [PubMed - as supplied by publisher]

Source: Protein and Peptide Letters - February 22, 2020 Category: Biochemistry Authors: Pandey G, Das PP, Ramakrishnan V Tags: Protein Pept Lett Source Type: research