Molecules, Vol. 25, Pages 1041: Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics

Molecules, Vol. 25, Pages 1041: Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics Molecules doi: 10.3390/molecules25051041 Authors: Ricardo Cervantes-Jiménez Lino Sánchez-Segura Laura Elena Estrada-Martínez Antonio Topete-Camacho Elizabeth Mendiola-Olaya Abraham Noé Rosas-Escareño Carlos Saldaña-Gutiérrez Mónica Eugenia Figueroa-Cabañas José Luis Dena-Beltrán Aarón Kuri-García Alejandro Blanco-Labra Teresa García-Gasca Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The aver...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research