Molecules, Vol. 25, Pages 1041: Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics
Molecules, Vol. 25, Pages 1041: Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics
Molecules doi: 10.3390/molecules25051041
Authors:
Ricardo Cervantes-Jiménez
Lino Sánchez-Segura
Laura Elena Estrada-Martínez
Antonio Topete-Camacho
Elizabeth Mendiola-Olaya
Abraham Noé Rosas-Escareño
Carlos Saldaña-Gutiérrez
Mónica Eugenia Figueroa-Cabañas
José Luis Dena-Beltrán
Aarón Kuri-García
Alejandro Blanco-Labra
Teresa García-Gasca
Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The aver...
Source: Molecules - Category: Chemistry Authors: Ricardo Cervantes-Jim énez Lino S ánchez-Segura Laura Elena Estrada-Mart ínez Antonio Topete-Camacho Elizabeth Mendiola-Olaya Abraham No é Rosas-Escareño Carlos Salda ña-Gutiérrez M ónica Eugenia Figueroa-Cabañas Jos é Luis Dena-Beltrán Aar ón Tags: Article Source Type: research
More News: Cancer | Cancer & Oncology | Carbohydrates | Chemistry | Colon Cancer | Colorectal Cancer | Men | Nanotechnology | Study