Intestinal Microbiome Dynamics in Solid Organ and Stem Cell Transplant Recipients
Conditions: Transplantation Infection; Kidney Transplant; Complications; Stem Cell Transplant Complications; Liver Transplant; Complications; Microbial Colonization Intervention: Diagnostic Test: Intestinal microbiome new generation sequencing Sponsor: Minsk Scientific-Practical Center for Surgery, Transplantation and Hematology Enrolling by invitation
Contributors : Elizabeth Ostrander ; Grant A ChallenSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusEpigenetic modifying enzymes DNMT3A and TET2 are recurrently mutated in hematological disorders despite possessing opposing biochemical functions in the DNA methylation processes. Using conditional ablation, we show these contrasting genotypes result in different functional effects in hematopoietic stem cells (HSCs). Loss of Dnmt3a bestows enhanced self-renewal on HSCs in serial, competitive repopulation assays while Tet...
Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive understanding of distinct subpopulations and their functional relevance in human satellite cells is lacking. We used a combination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically separate novel subpopulations of human PAX7+ satellite cells (Hu-MuSCs) from normal muscles. We found that, although relatively homogeneous compared to activated satellite cells and committed progenitors, the Hu-MuSC pool contains clusters of transcriptionally distinct cells with consistency across human individuals...
A 34-year-old man with chronic myelogenous leukemia status post allogeneic hematopoietic stem cell transplant (HSCT) on tacrolimus for graft-vs-host disease prophylaxis, presented with a 1-week history of fevers and severe headaches. Brain magnetic resonance imaging (MRI) demonstrated numerous small focal lesions in the left basal ganglia and left temporal lobe with a ring pattern, as well as a large lesion in the left occipital lobe with mild leptomeningeal enhancement (Figure 1). Cerebrospinal fluid (CSF) analysis revealed lymphocytic pleocytosis, and Toxoplasma gondii was detected by polymerase chain reaction (PCR).
Autologous induced pluripotent stem cells (iPSC)-based therapies are considered to be promising in the transplantation field, however their immunological potential is unclear. These therapies require expansion, reprogramming and differentiation of the cells in vitro. Mitochondrial (mt) DNA mutations can arise from those procedures and they have been considered to be involved in the rejection of the cells after transplantation. We therefore hypothesized that single nucleotide polymorphisms (SNPs) in the mtDNA can contribute to autologous and allogeneic graft immunoreactivity and we assessed their immunogenicity in transplantation.
Pulmonary chronic graft versus host disease (GVHD) is one of the thoracic manifestations that can complicate allogeneic hematopoietic stem cell transplantation (HSCT) causing respiratory failure and death. Lung transplantation (LTx) often remains the only therapeutic option for progressive forms although it has been reported in a small number of patients worldwide.
Our hypothesis is that human adipose mesenchymal stem cell (MSC)-derived extracellular vesicle (EV) can be used as a delivery platform for microRNAs to effectively attenuate lung ischemia-reperfusion injury (IRI).
This study aims to elucidate the efficacy of DPP4i in clinical LTx, and to search for a new biomarker for CLAD or overall survival (OS).
Transplantation of engineered heart tissue (EHT) derived from human induced pluripotent stem cells (hiPSC) represents a promising regenerative strategy. Recent studies provided first evidence that hiPSC-derived cardiomyocytes re-enter the cell cycle after transplantation. The aim of this project was to analyze cardiomyocyte proliferation after transplantation in detail.
Induced pluripotent stem cells (iPSCs) are promising candidates for regenerative cell-based therapies. Because autogenic iPSCs are not available for acute treatment procedures, allogeneic strategies have to be developed which evade immune rejection. We showed in this study that both mouse and human iPSCs lose their immunogenicity when major histocompatibility complex (MHC) class I and II genes are inactivated and CD47 is over-expressed.
Emerging evidence suggests that current xenograft valve replacements provoke an intense cell-mediated and humoral immune response that is a major regulator of time-dependent structural valve deterioration (SVD). Autologous recellularization may improve valvular durability by masking the xenograft scaffold from immune recognition. The purpose of this study was to directly address the role of autologous recellularization of xenograft scaffolds, in the regulation of the xenoreactive immune response in an in-vitro human model.