Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

Publication date: Available online 24 February 2020Source: Stem Cell ResearchAuthor(s): L.D. Shuvalova, A.V. Eremeev, A.N. Bogomazova, E.V. Novosadova, E.A. Zerkalenkova, Yu. V Olshanskaya, E.Y. Fedotova, E.S. Glagoleva, S.N. Illarioshkin, O.S. Lebedeva, M.A. LagarkovaAbstractIPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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Source: Stem Cell Research - Category: Stem Cells Source Type: research
Contributor : Yogita SharmaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSince the pioneering studies using fetal cell transplants in Parkinson ’s disease (PD), brain repair by cell replacement has remained a long-standing and realistic goal for the treatment of neurodegenerative disorders including PD. Authentic and functional dopamine (DA) neurons can now be generated from human pluripotent stem cells (hPSCs), and these cell preparation s are both safe and functional when transplanted to animal models of PD1. However, although resulting grafts from fetal brain tissue and hPS...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: International Journal of Medical Sciences - Category: Biomedical Science Tags: Int J Med Sci Source Type: research
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Source: Nature Reviews Neurology - Category: Neurology Authors: Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Current Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Curr Stem Cell Res Ther Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research
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