Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

Publication date: Available online 24 February 2020Source: Stem Cell ResearchAuthor(s): L.D. Shuvalova, A.V. Eremeev, A.N. Bogomazova, E.V. Novosadova, E.A. Zerkalenkova, Yu. V Olshanskaya, E.Y. Fedotova, E.S. Glagoleva, S.N. Illarioshkin, O.S. Lebedeva, M.A. LagarkovaAbstractIPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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Publication date: Available online 29 March 2020Source: Stem Cell ResearchAuthor(s): Muwan Chen, Muyesier Maimaitili, Susanne Hvolbøl Buchholdt, Uffe Birk Jensen, Fabia Febbraro, Mark Denham
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Contributor : Yogita SharmaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSince the pioneering studies using fetal cell transplants in Parkinson ’s disease (PD), brain repair by cell replacement has remained a long-standing and realistic goal for the treatment of neurodegenerative disorders including PD. Authentic and functional dopamine (DA) neurons can now be generated from human pluripotent stem cells (hPSCs), and these cell preparation s are both safe and functional when transplanted to animal models of PD1. However, although resulting grafts from fetal brain tissue and hPS...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
This study, for the first time, shows that transplantation of non-autologous mitochondria from healthy skeletal muscle cells into normal cardiomyocytes leads to short-term improvement of bioenergetics indicating "supercharged" state. However, over time these improved effects disappear, which suggests transplantation of mitochondria may have a potential application in settings where there is an acute stress. Outlining Some of the Science Behind Partial Reprogramming at Turn.bio https://www.fightaging.org/archives/2020/03/outlining-some-of-the-science-behind-partial-reprogramming-at-turn-bio/ Tur...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Authors: Tatullo M, Marrelli B, Zullo MJ, Codispoti B, Paduano F, Benincasa C, Fortunato F, Scacco S, Zavan B, Cocco T Abstract The scientific community continuously strives to get new disease models, to discover early markers or novel therapeutic approaches, improving the diagnosis and prognosis of several human pathologies. Parkinson's Disease (PD) is characterized by a long asymptomatic phase, characterized by a selective loss of dopaminergic neurons. Recently, the human Periapical Cyst-Mesenchymal Stem Cells (hPCy-MSCs) have been differentiated in functional dopaminergic neurons: such oral-derived MSCs and the ...
Source: International Journal of Medical Sciences - Category: Biomedical Science Tags: Int J Med Sci Source Type: research
Nature Reviews Neurology, Published online: 24 March 2020; doi:10.1038/s41582-020-0343-5An understanding of the biochemical processes underlying Parkinson disease (PD) will be essential for the development of disease-modifying therapies. In a recent study, a novel biochemical phenotype of the disease was identified from analysis of inducible pluripotent stem cell-derived dopaminergic neurons from individuals with young-onset PD.
Source: Nature Reviews Neurology - Category: Neurology Authors: Source Type: research
Publication date: Available online 17 March 2020Source: Stem Cell ResearchAuthor(s): Indrani Datta, Sowmithra, Soham Jagtap, Chandrakanta Potdar, Ravi Yadav, Pramod Pal
Source: Stem Cell Research - Category: Stem Cells Source Type: research
We report a new class of natural-product-inspired covalent inhibitors of telomerase that target the catalytic active site. Age-Related Epigenetic Changes that Suppress Mitochondrial Function https://www.fightaging.org/archives/2020/03/age-related-epigenetic-changes-that-suppress-mitochondrial-function/ Today's open access research reports on two specific epigenetic changes observed in old individuals that act to reduce mitochondrial function. This joins an existing list of genes for which expression changes are known to impact mitochondrial function with age. A herd of hundreds of mitochondria are found ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Abstract Stem cells are the undifferentiated cells in the body that possess the ability to differentiate and give rise to any type of cell in the body. In recent years, there has been a growing interest in therapy involving the stem cells as different treatment methods got developed. Depending on the source, there are two major kinds of stem cells, embryonic and adult stem cells. The former type is found in the embryo at the different developmental stages before the implantation and excels the latter owing to pluripotency. On the premise of the attributes of stem cells, they are touted as the "panacea for all...
Source: Current Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Curr Stem Cell Res Ther Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In this study, using a ratiometric bilirubin probe, we revealed that the mitochondrial inhibitor, rotenone, which is wi dely used to create a PD model, significantly decreased intracellular bilirubin levels in HepG2 cells. Chemical screening showed that BRUP‐1 was a top hit that restored cellular bilirubin levels that were lowered by rotenone. We found that BRUP‐1 up‐regulated the expression level of heme oxyge nase‐1 (HO‐1), one of the rate‐limiting enzyme of bilirubin production via nuclear factor erythroid 2‐related factor 2 (Nrf2) activation. In addition, we demonstrated that this Nrf2 activation was due ...
Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research
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