Anti-inflammatory effects of Lactobacillus johnsonii L531 in a pig model of Salmonella Infantis infection involves modulation of CCR6 + T cell responses and ER stress

AbstractProbiotic pretreatment is an effective non-antibiotic strategy for preventing or controllingSalmonella infections. We found thatLactobacillus johnsonii L531, isolated from the colon of a clinically healthy weaned piglet, effectively prevented infection withSalmonella enterica serovar Infantis in a pig model. Newly weaned piglets were intragastrically administeredLactobacillus johnsonii L531 at 1.0  × 1010 CFU/day for 1 week beforeS. Infantis challenge. Pretreatment withL. johnsonii L531 lessened the severity of diarrhea and ileal inflammation inS. Infantis –infected piglets. Lactobacilli were more abundant in the ileum than jejunum afterL. johnsonii L531 pretreatment. Treatment withL. johnsonii L531 reduced the abundance of total bacteria in the ileal mucosa and the production of lipocalin 2 in the jejunum of piglets challenged withSalmonella. Both intestinal morphology and transmission electron microscopy results indicated thatL. johnsonii L531 alleviated intestinal tissue damage followingS. Infantis challenge, especially in the villus and endoplasmic reticulum (ER). ER stress induced byS. Infantis was attenuated byL. johnsonii L531 treatment. The number of CD4− CCR6+ T cells decreased followingS. Infantis challenge, but the percentage of CCR6− IFN γ+ T cells in peripheral blood increased. In intestinal mesenteric lymph nodes,S. Infantis increased the proportion of CCR6+ IFN γ+ T cells, whereasL. johnsonii L531 induced an increase in the proportion of ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research