Development of RT-qPCR assays for the detection of three latent viruses of pome.

In this study we developed RT-qPCR assays for the detection of three latent viruses of pome, Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), and Apple mosaic virus (ApMV), using the alignment of representative sequences from the NCBI database. The optimized assays demonstrated specificity by successfully amplifying the target from positive controls without showing any detectable amplification in negative and non-target controls, and revealed high sensitivity by reliably detecting as low as 101 copies per reaction. The assays also demonstrated that both the choice of extraction method and the reagents used for RT-qPCR can play a critical role in virus detection outcome. These assays are both reliable and robust compared to extant RT-PCR methods, and they are a viable tool on making informed management decisions. PMID: 32087190 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research