Hydroxyl groups in cosolutes regulate the G-quadruplex topology of telomeric DNA.

In this study, we quantitatively investigated the effect of cosolutes, particularly the varying number of hydroxyl groups, on the structural transition between hybrid type and parallel G-quadruplexes formed by telomeric DNA sequences. Cosolutes with one or no hydroxyl groups in the vicinal position more efficiently induced the transition to parallel G-quadruplex from hybrid G-quadruplex than those with more hydroxyl groups. We also examined the effect of cosolute structures on the hydration of G-quadruplex formation; the results indicated that cosolutes with fewer hydroxyl groups lead to the release of greater amount of water during G-quadruplex formation. Molecular dynamics results showed that the parallel G-quadruplex was more dehydrated than the hybrid type G-quadruplex. Generally, a dehydrated structure is favored under crowding condition. Thus, depending on the surrounding cosolutes, the G-quadruplex topology can be controlled by the G-quadruplex hydration state.
PMID: 32081425 [PubMed - as supplied by publisher]

Source: Biochemical and Biophysical Research communications - February 17, 2020 Category: Biochemistry Authors: Tateishi-Karimata H, Banerjee D, Ohyama T, Matsumoto S, Miyoshi D, Nakano SI, Sugimoto N Tags: Biochem Biophys Res Commun Source Type: research