Development and in vitro characterization of diazepam loaded PLA nanoparticles

In this study we tried to explore Diazepam as a repurposed drug and tested it in Neuroblastoma (Neuro 2a) cells. Diazepam loaded Poly Lactic Acid Nanoparticles were developed (D-PLA-NPs) using nanoprecipitation method. Entrapment efficiency was found to be ± 99.7% and drug loading was ± 12.88%. The particle size developed was 188.8 nm and zeta potential was −25.6 millivolt. Dissolution profiles were studied in PBS (pH 7.4) or simulated media, almost 50% release was observed within 6 h. In vitro, cytotoxicity was analyzed using MTT assay where dose-dependent cytotoxicity was observed for developed D-PLA-NPs.
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research

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Authors: Roşu GA, Furău GO, Ionescu CA, Dimitriu MCT, Neacşu A, Ioan RG, Călin FD, Matei A, Bănacu M, Popescu I, Gheorghiu D, Neacşu I, Bacalbaşa N, Furău CG Abstract Epithelial ovarian carcinoma makes up 90-95% of all ovarian malignancies, taking into account also low-malignant-potential tumors. The Krukenberg tumor is a rare metastatic adenocarcinoma (ADK) in the ovary, representing 1-2% of ovarian tumors. Multiple primary malignant neoplasms may exist when more than one cancerous tumor is diagnosed in the same or a different organ. The incidence of multiple primary cancers among malignancies is between 2...
Source: Romanian Journal of Morphology and Embryology - Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research
Despite intensive multimodal therapy, the survival rate for high risk neuroblastoma (HR-NB) remains
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Source: Cancer Management and Research - Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research
We identified 20 survival ‐related long noncoding RNAs (lncRNAs) that might be associated with the spontaneous regression of neuroblastoma (NB) and developed a four‐lncRNA signature risk score. The four‐lncRNA signature is an independent prognostic factor of survival in NB. AbstractBackgroundThe association between long noncoding RNAs (lncRNAs) and spontaneous regression of neuroblastoma (NB) has rarely been investigated and remains unknown.ObjectiveTo identify prognostic lncRNAs involved in the spontaneous regression of NB.MethodsDifferential expression analyses were performed between those samples with an outcome o...
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research
Neuroblastoma (NB) tumor rupture is a rare oncology emergency with a poor prognosis. We aimed to evaluate patient clinical characteristics and risk factors for ruptured NB.
Source: BMC Cancer - Category: Cancer & Oncology Authors: Tags: Research article Source Type: research
Contributors : Tao Liu ; Andrew Tee ; Matthew Wong ; Nicolas HoSeries Type : Expression profiling by arrayOrganism : Homo sapiensThe CDK7 inhibitor THZ1 has been shown to suppress MYCN gene transcription but not cause significant cell death as a single agent.The tyrosine kinase inhibitors (TKIs) ponatinib and lapatinib were found to exert synergistic anti-cancer effects in combination with the CDK7 inhibitor THZ1, on MYCN amplified neuroblastoma cell lines.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
GPAID was designed to treat high ‐risk neuroblastoma, including relapsed or refractory disease. GPAID targets neuroblastoma cells in the same way as MIBG. It also cotargets DNA of proliferating cells, an attribute especially advantageous in the treatment ofMYCN‐amplified tumors. Neuroblastoma, the most common extracranial solid tumor in children, accounts for nearly 8% of childhood cancers in the United States. It is a disease with pronounced clinical and biological heterogeneities. The amplification ofMYCN, whose key tumorigenic functions include the promotion of proliferation, facilitation of the cell's entry into th...
Source: Journal of Labelled Compounds and Radiopharmaceuticals - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the r...
Source: Journal of Experimental and Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
CONCLUSION: The five m6A regulatory genes identified in this study enable clinical prognosis of NB and may serve as novel therapeutic targets for NB. PMID: 32176634 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
StC. Black The dihydropyranoindole scaffold was identified as a promising target for improving the anti-cancer activity of HDAC inhibitors from the preliminary screening of a library of compounds. A suitable methodology has been developed for the preparation of novel dihydropyranoindoles via the Hemetsberger indole synthesis using azido-phenylacrylates, derived from the reaction of corresponding alkynyl-benzaldehydes with methyl azidoacetate, followed by thermal cyclization in high boiling solvents. Anti-cancer activity of all the newly synthesized compounds was evaluated against the SH-SY5Y and Kelly neuroblastoma ce...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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