Rapid determination of isoflavones and other bioactive compounds in soybean using SWATH-MS.

In this study, a high-throughput SWATH-MS method for the determination of 12 isoflavones in soybeans was established. Furthermore, amino acids, saponins can be semi-quantitated from the same SWATH-MS data. Combination of targeted quantification and untargeted profiling with SWATH, all bioactive compounds were analyzed within 5 min in 10 min run time, and the method had good linear regression with r2 > 0.99. The precisions (RSD %) of the intra-day and inter-day analyses ranged from 2.11% to 18.7%, and the accuracies (RE%) ranged from -14.39% to 17.48%. The matrix effect ranged from 88.66% to 114.82%. Moreover, 7 varieties of soybeans were analyzed and compared with this robust screening method.
PMID: 32081177 [PubMed - in process]

Source: Analytica Chimica Acta - February 23, 2020 Category: Chemistry Authors: Chien HJ, Wang CS, Chen YH, Toh JT, Zheng YF, Hong XG, Lin HY, Lai CC Tags: Anal Chim Acta Source Type: research