A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples.

A sensitive LC-MS assay using derivatization with boron trifluoride to quantify curcuminoids in biological samples. Anal Biochem. 2020 Feb 17;:113636 Authors: Yoon AJ, Wu H, Pan RD, Teter B, Cipolla J, Chang E, Avila LZ, Basak SK, Srivatsan E, Wang MB, Cole GM, Frautschy SA, Hampton PD, Faull KF Abstract A procedure is described to measure curcumin (C), demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC), tetrahydrocurcumim (TC) and their glucuronidated metabolites (CG, DMCG, and BDMCG) in plasma, brain, liver and tumor samples. The procedure involves converting the analytes to their boron difluoride derivatives and analyzing them by combined liquid chromatography coupled to an ion trap mass spectrometer operating in the negative ion MSn scan mode. The method has superb limits of detection of 0.01 nM for all curcuminoids and 0.5 nM for TC and the glucuroniated metabolites, and several representative chromatograms of biological samples containing these analytes are provided. In addition, the pharmacokinetic profile of these compounds in one human who daily consumed an over-the-counter curcuminoid product shows the peak and changes in circulating concentrations achieved by this mode of administration. PMID: 32081619 [PubMed - as supplied by publisher]
Source: Analytical Biochemistry - Category: Biochemistry Authors: Tags: Anal Biochem Source Type: research