Effect of olfactory ensheathing cells combined with chitosan on inhibition of P2×4 receptor over-expression-mediated neuropathic pain

In this study, olfactory ensheathing cells (OECs) were cultured, chitosan (CS) was prepared, and the compatibility of CS and OECs was detected by MTT method. Animal model of chronic constrictive sciatic nerve injury (CCI) was made, OECs and OECs+CS were transplanted to the region surrounding the chronic sciatic nerve injury, and the difference between the two groups in the treatment of NPP was compared. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured by using behavioral method. In situ hybridization and Western-blotting were used to detect the expression of P2X4R mRNA and protein in the DRG. These results showed that OECs had good biocompatibility with CS. Compared with the CCI, the MWT and TWL were significantly increased (P<0.05), the expression levels of P2X4R mRNA and protein in the OECs and OECs+CS group were significantly reduced (P<0.05). Compared with the OECs, the expression levels of P2X4R mRNA and protein in the OECs+CS group were significantly reduced (P<0.05), the MWT and TWL were significantly increased (P<0.05). We conclude that OECs+CS can better inhibit P2X4R over-expression-mediated NPP, and its therapeutic effect was superior to simple OECs transplantation, which may become another potential method for the treatment of NPP.
Source: Neuroscience Letters - Category: Neuroscience Source Type: research

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CONCLUSIONS: In our small series of patients with refractory chronic SUNCT/SUNA, SPG-PRF was a safe and effective treatment modality. The potential reproducible positive effect of subsequent treatments may prevent or delay the use of more invasive and costly interventions for at least a proportion of these patients. PMID: 32202666 [PubMed - as supplied by publisher]
Source: Headache - Category: Neurology Authors: Tags: Headache Source Type: research
Toll-like receptor 7 contributes to neuropathic pain by activating NF-κB in primary sensory neurons. Brain Behav Immun. 2020 Mar 20;: Authors: He L, Han G, Wu S, Du S, Zhang Y, Liu W, Jiang B, Zhang L, Xia S, Jia S, Hannaford S, Xu Y, Tao YX Abstract Toll like receptor 7 (TLR7) is expressed in neurons of the dorsal root ganglion (DRG), but whether it contributes to neuropathic pain is elusive. We found that peripheral nerve injury caused by ligation of the fourth lumbar (L4) spinal nerve (SNL) or chronic constriction injury of sciatic nerve led to a significant increase in the expression of TLR7...
Source: Brain, Behavior, and Immunity - Category: Neurology Authors: Tags: Brain Behav Immun Source Type: research
This study aims to characterise the CSF cellular and peptide constituents in patients with CNP and the effect of pulsed radiofrequency (PRF) on these constituents and reported pain.
Source: Journal of Neuroimmunology - Category: Allergy & Immunology Authors: Source Type: research
We examined its role in inflammatory pain and demonstrated that its expression changed in different neuronal subpopulations of the dorsal root ganglia at different times after cutaneous inflammation. Furthermore, this receptor wo rks co‐operatively with the type‐1 receptor for angiotensin II via regulation of the neuritogenic activity of Angiotensin II on non‐peptidergic isolectin B4 binding and peptidergic trkA‐expressing dorsal root ganglion neurons. These findings have important implications for the clinical use o f angiotensin‐II receptor blockers in the treatment of pain.Image content: Triple immunofluoresce...
Source: Journal of Neurochemistry - Category: Neuroscience Tags: Issue Cover Source Type: research
ConclusionsDRG and SCS are cost ‐effective treatments for chronic pain secondary to CRPS‐I and II compared to CMM. DRG accrued higher cost due to higher conversion from trial to permanent implant and shorter battery life, but DRG was the most beneficial therapy due to more patients receiving permanent implants and experiencing higher quality of life compared to SCS. New DRG technology has improved battery life, which we expect to make DRG more cost‐effective compared to both CMM and SCS in the future.
Source: Neuromodulation: Technology at the Neural Interface - Category: Biotechnology Authors: Tags: Clinical Research Source Type: research
Neuromodulation: Technology at the Neural Interface, EarlyView.
Source: Neuromodulation: Technology at the Neural Interface - Category: Biotechnology Authors: Tags: Letter to the Editor Source Type: research
This study aimed to test the hypothesis that miR‐34c‐5p can modulate neuropathic pain in rat models with chronic constriction injury (CCI) of sciatic nerve,via interaction with the SIRT1/STAT3 signaling pathway Firstly, SIRT1 was validated as a target gene of miR ‐34c‐5p and could be negatively regulated by miR‐34c‐5p. We induced miR‐34c‐5p overexpression/inhibition, SIRT1 knockdown, and STAT3 knockdown in the model rats to assess pain behavior patterns. Meanwhile, dorsal root ganglion (DRG) was transduced with overexpression or knockdown of miR‐ 34c‐5p or lipopolysaccharide to induce the production of ...
Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research
Trigeminal neuralgia is one of the most common of the neuropathic pains, and it can seriously influence patients ’ quality of life. Calcitonin gene-related peptide (CGRP) is a type of nociceptive neurotransmitter that is expressed in neurons of the trigeminal ganglion and plays a major part in transmitting pain. The rat model of trigeminal neuralgia was established by causing a chronic constriction injury of the infraorbital nerve (CCI-ION). Male Sprague-Dawley rats (n=24) were randomly divided into a sham control group (sham, n=6), sham-treated with palmatine group (sham+palmatine, n=6), trigeminal nerve model group...
Source: The British Journal of Oral and Maxillofacial Surgery - Category: ENT & OMF Authors: Tags: Basic Science Article Source Type: research
Conclusion: We identified a few GOs, pathways, and genes that could play key roles in the amelioration of CIP by EA. Hence, this study may provide a theoretical basis for CIP amelioration by EA. PMID: 32104194 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Conclusions: B-vitamin treatment can greatly suppress chronic DNP and DNP-associated increased activities of P2X3 and TRPV1 in DRG and the spinal proinflammatory cytokines, which may contribute to the pathogenesis of DNP. Systematic administration of B vitamins can be a strategy for DNP management in clinic. PMID: 32104520 [PubMed - in process]
Source: Pain Research and Management - Category: Anesthesiology Authors: Tags: Pain Res Manag Source Type: research
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