Polyaniline stabilized Silver (I) Oxide nanocubes for sensitive and selective detection of hemoglobin in urine for hematuria evaluation

In this report, the Ag2O nanocubes were synthesized by the co-precipitation method and further stabilized with polyaniline (PANI). The bio-complex formed between PANI stabilized Ag2O nanocubes, and heme group of Hemoglobin (Hb) resulted in an increase of absorption intensity of Ag2O nanocubes at 420 nm. This is due to the electronic transition from Heme to Ag+. This also leads to conformational changes in Hb. Ag2O-PANI interactions and stability studies were confirmed by Ultraviolet-Visible spectroscopy (UV–Vis), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Raman spectroscopy. The morphology of Ag2O nanocubes was confirmed by Transmission electron Microscopy (TEM). The interaction between Ag2O-PANI and Hb was confirmed by UV–Vis spectroscopy. The current assay is very sensitive for hematuria with an extremely low detection limit of 5.35 × 10−13 M from urine.
Source: Microchemical Journal - Category: Chemistry Source Type: research