Diets with Low n-6:n-3 PUFA Ratio Protects Rats from Fructose-Induced Dyslipidemia and Associated Hepatic Changes: Comparison between 18:3 n-3 and Long-Chain n-3 PUFA

Publication date: Available online 22 February 2020Source: Prostaglandins, Leukotrienes and Essential Fatty AcidsAuthor(s): Anil Sakamuri, Siva S.V.P. Sakamuri, Suryam Reddy Kona, Sugeedha Jayapal, Ahamed IbrahimAbstractIn the present study, we investigated the impact of substituting alpha-linolenic acid (ALA) or long-chain n-3 PUFA (eicosapentaenoic acid and docosahexaenoic acid) for linoleic acid and hence decreasing n-6:n-3 PUFA ratio on high-fructose diet-induced hypertriglyceridemia and associated hepatic changes. Weanling male Wistar rats were divided into four groups and fed with starch-diet (n-6:n-3 PUFA ratio 215:1) and high-fructose diets with different n-6:n-3 PUFA ratio (215:1, 2:1 with ALA and 5:1 with long-chain n-3 PUFA) for twenty-four weeks. Substitution of linoleic acid with ALA (n-6:n-3 PUFA ratio of 2) or long-chain n-3 PUFA (n-6:n-3 PUFA ratio of 5) protected the rats from fructose-induced dyslipidemia, hepatic oxidative stress and corrected lipogenic and proinflammatory gene expression. Both ALA and long-chain n-3 PUFA supplementation also reversed the fructose-induced upregulation of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) gene, which is involved in the generation of active glucocorticoids in tissues. Although both ALA and LC n-3 PUFA prevented fructose-induced dyslipidemia to a similar extent, compared to ALA, LC n-3 PUFA is more effective in preventing hepatic oxidative stress and inflammation.Graphical abstract

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - February 22, 2020 Category: Lipidology Source Type: research