Purification and characterization of antimicrobial peptide fractions of Junipers seravschanica

Publication date: Available online 21 February 2020Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Khamis Al-Dhafri, Chai Lay Ching, Saiful Anuar KarsaniAbstractThe emergence of antibiotic resistance among bacteria leads to the urge of finding an alternative solution. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. This research was designed to explore the potency of antimicrobial peptides from Omani Juniperus seravschanica plant and their mode of action. The isolated AMPs showed to have inhibitory activity towards pathogenic bacteria namely Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, Methicillin-resistant Staphylococcus aureus (MRSA) and fungi such as Aspergillus flavus, and Cladosporium herbarum. The isolated AMPs showed noticeable changes in OD, illustrating clearly the pore formation. Scanning electron microscope showed significant membrane disruption of the bacteria. The three AMP fractions displayed to be heat stable and functioned well at low pH (2–6). Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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François Chassagne1†, Xinyi Huang1†, James T. Lyles1 and Cassandra L. Quave1,2* 1Center for the Study of Human Health, Emory University, Atlanta, GA, United States 2Department of Dermatology, Emory University, Atlanta, GA, United States In the search for new therapeutic solutions to address an increasing number of multidrug-resistant bacterial pathogens, secondary metabolites from plants have proven to be a rich source of antimicrobial compounds. Ginkgo biloba, a tree native to China, has been spread around the world as an ornamental tree. Its seeds have been used as snacks and medical mater...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion: The River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated. Introduction Nature is an exceptional resource of new biologically active compounds with an astonishing chemical diversity such as that found in plant species, animals, and microorganisms (Butler et al., 2014). Microbial diversity constitutes an infinite pool of novel chemistry, making up a valuable source for innovative biotechnology (Bérdy, 2005; Fenical and Jensen, 2006). The aquatic environment is now becoming more appreciated as a ri...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Raquel Ferrer-Espada1,2, Xiaojing Liu1,2, Xueping Sharon Goh1,2 and Tianhong Dai1,2* 1Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States 2Vaccine &Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States Polymicrobial biofilms, in which mixed microbial species are present, play a significant role in persistent infections. Furthermore, polymicrobial biofilms promote antibiotic resistance by allowing interspecies transfer of antibiotic resistance genes. In the present study, we investigated the effec...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study aimed to contribute to the need of finding new and more effective bioactive molecules against several of the earlier mentioned threats faced by human kind nowadays. For this, eighteen species from the Actinomycetales previously isolated from Erylus spp. sponges collected in Portuguese marine waters (Açores, Madeira, and continental shelf) were screened for antimicrobial, anti-cancer, anti-parasitic and anti-obesogenic activities. Materials and Methods Biological Material The bacteria under study belong to the order Actinomycetales within the phylum Actinobacteria and were isolated from marine sponges o...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: June 2018Source: Biotechnology Reports, Volume 18Author(s): Mostafa M. Abo Elsoud, Ola E.A. Al-Hagar, Eman S. Abdelkhalek, N.M. SidkeyAbstractTellurium has attracted the attention of many researchers and manufacturers due to its unique properties. Through the current work, six fungal isolates have been screened for their ability to reduce potassium tellurite (K2TeO3) into elemental tellurium nanoparticles (TeNPs). The most promising fungal isolate was identified as Aspergillus welwitschiae and given the accession number (KY766958) based on molecular basis and has been used for biogenic (enzymatic) product...
Source: Biotechnology Reports - Category: Biotechnology Source Type: research
Publication date: 15 September 2017 Source:LWT - Food Science and Technology, Volume 83 Author(s): Surya Kant Verma, Shiv Kumar Sood, Ram Krishan Saini, Neha Saini The present study was carried out to enhance shelf life of raw buffalo milk by the use of pediocin PA-1 containing fermented cheese whey (PCFCW) as bio-preservative. Ten distinct genera of milk microbes could be identified as Streptococcus, Enterococcus, Salmonella, Campylobacter, Lactobacillus, Enterobacter, E. coli, Shigella, Candida and Aspergillus. For the production of PCFCW, supplemented cheese whey medium was fermented with Pediococcus pentosaceous NCDC ...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Abstract Lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (CF). N-chlorotaurine (NCT), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. The aim of this study was to investigate NCT on its bactericidal and fungicidal activity in vitro in artificial sputum medium (ASM) mimicking the composition of cystic fibrosis mucus.The medium was inoculated with bacteria (Staphylococcus aureus including some MRSA strains, Pseudomonas aeruginosa, Escherichia coli) or spores of fungi (Aspergillus fumigatus, Aspergi...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
ConclusionPKS gene expression indicated β keto‐acyl synthase as one of the important active domains in the biomolecules related to antitumour and antifungal group. The simultaneous identification of the biomolecule (dihydro‐2H‐pyran‐2‐one derivative) was also inferred spectroscopically. Significance and impact of the studyFreshwater cyanobacteria are the prolific producers of secondary metabolite(s) that may act as the antimicrobial drug resource in addition to their much explored marine counterpart.This article is protected by copyright. All rights reserved.
Source: Journal of Applied Microbiology - Category: Microbiology Authors: Tags: Original Article Source Type: research
Publication date: 1 November 2016 Source:Materials Science and Engineering: C, Volume 68 Author(s): S.A. Abdel Moaty, A.A. Farghali, Rehab Khaled Facile and simple processes to get Zn-Fe layered double hydroxide (LDH) with nitrate as the interlayer anion are reported. The method of co-precipitation produced high crystallinity LDH that is marked by XRD, SEM, TEM and FT-IR. Results showed that 99.8% of Cd+2 removals were at pH11 and 4h. To get the adsorption isotherms, the concentration of metal ions extending from 6 to 18mg/L was utilized. Results supported the Langmuir adsorption model. In contrary, the adsorption pro...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Authors: Ogidi OC, Oyetayo VO, Akinyele BJ Abstract In recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. The phytochemistry as well as antimicrobial activity of extracts obtained from Lenzites quercina was investigated. The extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. The quantity of phytochemicals found in the extracts of L. quercina ranged from 14.4 to 20.7 mg/g for alkaloids,...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
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