Detection and monitoring of lineage-specific chimerism by digital droplet PCR-based testing of deletion/insertion polymorphisms

Molecular monitoring of hematopoietic chimerism is an important part of the routine diagnostic program in patients after allogeneic stem cell transplantation (SCT). Common employment of reduced-intensity conditioning (RIC) regimens and cord blood (CB) transplants, which require very careful surveillance of the graft, has further increased the clinical importance of chimerism analysis [1,2]. Chimerism testing facilitates early prediction and documentation of successful engraftment, and permits early risk assessment for impending graft failure or rejection [3-9].
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research