Multifaceted secretion of htNSC-derived hypothalamic islets induces survival and antidiabetic effect via peripheral implantation in mice

We report that mouse hypothalamic stem/progenitor cells produce multiple pancreatic, gastrointestinal and hypothalamic peptides in addition to exosomes. Through cell sorting and selection according to insulin promoter activity, we generated a subpopulation of these cells which formed 3D spherical structure with combined features of hypothalamic neurospheres and pancreatic islets. Through testing streptozotocin-induced pancreatic islet disruption and fatal diabetes, we found that peripheral implantation of these spheres in mice led to remarkable improvements in general health and survival in addition to a moderate antidiabetic effect, and notably these pro-survival versus metabolic effects were dissociable to a significant extent. Mechanistically, secretion of exosomes by these spheres was essential for enhancing survival while production of insulin was important for the antidiabetic effect. In summary, hypothalamic neural stem/progenitor cells comprise subpopulations with multifaceted secretion, and this subpopulation-derived hypothalamic islets can be implanted peripherally to enhance general health and survival together with an antidiabetic benefit.
Source: eLife - Category: Biomedical Science Tags: Stem Cells and Regenerative Medicine Source Type: research

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Publication date: Available online 8 October 2020Source: Stem Cell ResearchAuthor(s): Edward Po-Fan Chu, Chia-Hung Lin, Candy Hsin-Hua Cho, I-Fen Cheng, Tzu-Chien Kuo, Ruei-Ying Chen, Chia-Nan Liao, Jen-Chien Cheng, Jason Isheng Tsai, Po-Chuan Wang, Shing-Jyh Chang, Chia-Ning Shen
Source: Stem Cell Research - Category: Stem Cells Source Type: research
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Source: Translational Research - Category: Research Authors: Tags: Original Research Article Source Type: research
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Source: Journal of Molecular Medicine - Category: Molecular Biology Source Type: research
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Source: Stem Cells and Development - Category: Stem Cells Authors: Source Type: research
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Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: https://www.fightaging.org/newsletter/ Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
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Source: International Journal of Molecular Medicine - Category: Molecular Biology Authors: Tags: Int J Mol Med Source Type: research
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