Genes, Vol. 11, Pages 227: Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma

Genes, Vol. 11, Pages 227: Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma Genes doi: 10.3390/genes11020227 Authors: Qi Wan Xuan Sang Lin Jin Zhichong Wang Growing evidence has revealed that abnormal alternative splicing (AS) events are closely related to carcinogenic processes. However, the comprehensive study on the prognostic value of splicing events involved in uveal melanoma (UM) is still lacking. Therefore, splicing data of 80 UM patients were obtained from the Cancer Genome Atlas (TCGA) SpliceSeq and RNA sequence data of UM and patient clinical features were downloaded from the Cancer Genome Atlas (TCGA) database to identify survival related splicing events in UM. As a result, a total of 37996 AS events of 17911 genes in UM were detected, among which 5299 AS events of 3529 genes were significantly associated with UM patients’ survival. Functional enrichment analysis revealed that this survival related splicing genes are corelated with mRNA catabolic process and ribosome pathway. Based on survival related splicing events, seven types of prognostic markers and the final overall prognostic signature could independently predict the overall survival of UM patients. Finally, an 11 spliced gene was identified in the final signature. On the basis of these 11 genes, we constructed a Support Vector Machine (SVM) classifier and evaluated it with leave-one-out cross-validation. The results showed that the 11 genes could det...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research