Prophylaxis of implant-related infections by local release of vancomycin from a hydrogel in rabbits.

Prophylaxis of implant-related infections by local release of vancomycin from a hydrogel in rabbits. Eur Cell Mater. 2020 Feb 19;39:108-120 Authors: Boot W, Vogely HC, Jiao C, Nikkels PG, Pouran B, van Rijen MH, Ekkelenkamp MB, Hänsch GM, Dhert WJ, Gawlitta D Abstract Local prophylaxis with antibiotic-loaded bone cement is a successful method to prevent post-operative infections in patients receiving orthopaedic implants. No comparable method is available for uncemented implants. Therefore, a hydrogel consisting of hyaluronic and polylactic acids was evaluated in a rabbit model for delivery of antimicrobial agents to prevent post-operative infections. In a pilot study, the suitability of the in vivo model was assessed by testing the hydrogel as carrier material for antimicrobial agents.In the main study, the antimicrobial-agent-loaded hydrogel was evaluated for infection prophylaxis. Rabbits received a titanium rod intramedullary in the tibia after contamination with Staphylococcus aureus. The rods were coated with unloaded hydrogel (Gel), hydrogel loaded with 2 % (Van2) or 5 % vancomycin (Van5), bioactive glass (BAG) or N-acetyl-L-cysteine (NAC). To analyse the infection severity after 28 d, histopathological, bacteriological, micro-computed tomographic and haematological analyses were performed. In the pilot study, the Van5 group had less infection (0/6 infected) as compared to the Gel group (5/5, p = 0.000) and the in vivo model ...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research