Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model.

Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model. Toxicon. 2020 Feb 14;: Authors: Coelho Thomazi GO, da Costa A, Rodrigues JP, Alves GJ, Prezotto Neto JP, de Oliveira Turíbio T, Rocha AM, da Silva Aires R, Seibert CS, Spencer PJ, Galisteo Júnior AJ, de Andrade Júnior HF, do Nascimento N Abstract Accidents by freshwater stingrays are common in northern Brazil, there is no specific therapy for high morbidity and local tissue destruction. The irradiation of venoms and toxins by ionizing radiation has been used to produce appropriate immunogens for the production of antisera. We planned to study the efficacy of stinging mucus irradiation in the production of antisera, with serum neutralization assays of edematogenic activity and quantification of cytokines performed in animal models of immunization with native and irradiated mucus of Paratrygon aiereba, a large freshwater stingray. Antiserum potency and its cross-reactivity with mucus from other freshwater stingrays were detected by ELISA. Immunization models demonstrated the ability to stimulate a strong humoral response with elevated levels of serum IgG detectable by ELISA, and both native and irradiated mucus were immunogenic and capable of recognizing mucus proteins from other freshwater neotropical stingrays. Mucus P. aiereba causes cellular and humoral adaptive immune responses in cells of immunized mice producing antibodi...
Source: Toxicon - Category: Toxicology Authors: Tags: Toxicon Source Type: research