LncRNA MALAT1 regulates diabetic cardiac fibroblasts through the Hippo/YAP signaling pathway.

In this study, cardiac fibroblasts (CFs) were transfected with si-MALAT1 and exposed to high glucose. CFs in high glucose groups were treated with 30 mmoL/L glucose and control CFs were treated with 5.5 mmoL/L glucose. MALAT1 expression in the nucleus and cytoplasm of CFs was detected. CF biological behaviors and collagen production, Hippo/YAP pathway and YAP nuclear localization were measured. DCM mouse models were established to observe pathological changes of myocardium and determine levels of collagen I, Bax and Bcl-2. The interaction between MALAT1 and YAP was analyzed and CREB expression in high-glucose CFs was detected. MALAT1 was upregulated in high-glucose CFs and located in the nucleus. High-glucose increased collagen production, inflammation, cell proliferation and invasion, MST1 and LATS1 phosphorylation and promoted YAP nuclear translocation. These trends in high-glucose CFs and DCM mice were reversed by si-MALAT1. MALAT1 positively regulated YAP nuclear translocation by binding to CREB. CREB was increased in high-glucose CFs but decreased after silencing MALAT1. Taken together, si-MALAT1 reduces inflammation and collagen accumulation in high-glucose CFs and DCM mice via the Hippo/YAP pathway and CREB. PMID: 32069074 [PubMed - as supplied by publisher]
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research