Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use

Publication date: Available online 20 February 2020Source: Biosensors and BioelectronicsAuthor(s): Lung-Chieh Chen, Erick Wang, Chun-San Tai, Yuan-Chen Chiu, Chang-Wei Li, Yan-Ren Lin, Tsung-Han Lee, Ching-Wen Huang, Jung-Chih Chen, Wen Liang ChenAbstractElectrochemical biosensors possess numerous desirable qualities for target detection, such as portability and ease of use, and are often considered for point-of-care (POC) development. Label-free affinity electrochemical biosensors constructed with semiconductor manufacturing technology (SMT)-produced electrodes and a streptavidin biomediator currently display the highest reproducibility, accuracy, and stability in modern biosensors. However, such biosensors still do not meet POC guidelines regarding these three characteristics. The purpose of this research was to resolve the limitations in reproducibility and accuracy caused by problems with production of the biosensors, with the aim of developing a platform capable of producing devices that exceed POC standards. SMT production settings were optimized and bioreceptor immobilization was improved through the use of a unique linker, producing a biosensor with exceptional reproducibility, impressive accuracy, and high stability. Importantly, the three characteristics of the sensors produced using the proposed platform all meet POC standards set by the Clinical and Laboratory Standards Institute (CLSI). This suggests possible approval of the biosensors for POC development. Furthe...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research