Titration methods for rVSV-based vaccine manufacturing

In this study, we describe the titration tools elaborated to assess the titre of rVSV-ZEBOV productions.- A streamlined Median Tissue Culture Infectious Dose (TCID50) assay to determine the infectious titer of this vaccine was established.- A digital polymerase chain reaction (dPCR) assay to assess the total number of viral particles present in cell-free culture supernatants of rVSV productions was developed.- These assays are used to titre rVSV-ZEBOV samples and characterize the ratio of total particles to infectious units for monitoring process robustness and product quality attributes and can be used to titre samples generated in the production of further rVSV vectors.Graphical abstractGraphical abstract
Source: MethodsX - Category: Science Source Type: research