Magnitude of modulation of gene expression in aneuploid maize depends on the extent of genomic imbalance

Publication date: Available online 19 February 2020Source: Journal of Genetics and GenomicsAuthor(s): Adam F. Johnson, Jie Hou, Hua Yang, Xiaowen Shi, Chen Chen, Md Soliman Islam, Tieming Ji, Jianlin Cheng, James A. BirchlerAbstractAneuploidy has profound effects on an organism, typically more so than polyploidy, and the basis of this contrast is not fully understood. A dosage series of the maize long arm of chromosome 1 (1L) was used to compare relative global gene expression in different types and degrees of aneuploidy to gain insights into how the magnitude of genomic imbalance as well as hypoploidy affects global gene expression. While previously available methods require a selective examination of specific genes, RNA sequencing provides a whole-genome view of gene expression in aneuploids. Most studies of global aneuploid effects have concentrated on individual types of aneuploids because multiple dose aneuploids of the same genomic region are difficult to produce in most model genetic organisms. The genetic toolkit of maize allows the examination of multiple ploidies and 1‒4 doses of chromosome arms. Thus, a detailed examination of expression changes both on the varied chromosome arms and elsewhere in the genome is possible, in both hypoploids and hyperploids, compared with euploid controls. Previous studies observed the inverse trans effect, in which genes not varied in DNA dosage are expressed in a negative relationship to the varied chromosomal region. This respons...
Source: Journal of Genetics and Genomics - Category: Genetics & Stem Cells Source Type: research
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