CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR.

CAT25 defines microsatellite instability in colorectal cancer by high-resolution melting PCR. Br J Biomed Sci. 2020 Feb 17; Authors: Sánchez AG, Juaneda I, Eynard H, Basquiera AL, Palazzo E, Calafat P, Palla V, Romagnoli PA, Alvarellos T Abstract Background: CAT25 (T25mononucleotide repeat of the Caspase 2 gene), is a promising DNA marker for detecting microsatellite instability (MSI) in colorectal cancer. CAT25 has the potential to be incorporated into the Bethesda panel, a commonly used panel of DNA microsatellites, or replace it in its entirety. We aimed to develop and validate a high-resolution melting-PCR (HRM-PCR) method for CAT25 instability detection in clinical samples and study its allelic variation in our population.Methods: The instability of CAT25, BAT25 (a poly(A) tract occurring in the c-kit gene) and BAT26 (a poly(A) tract localized in the hMSH2 gene) microsatellites was assessed in DNA from tumour and peripheral blood obtained from 110 patients with colorectal cancer using HRM-PCR and capillary electrophoresis. Immunohistochemistry (IHC) staining for MSH2, MSH6, MLH1, and PMS2 enzymes was performed on tumours with high MSI. Allelic size variation of CAT25 was analyzed on peripheral blood DNA from 208 healthy volunteers.Results: The HRM-PCR for CAT25 was validated in clinical samples. CAT25 showed a tight range of 64-66 base pairs. Of 110 tumours, 11 had high MSI, later confirmed by IHC. CAT25 defined MSI alone and when used togethe...
Source: British Journal of Biomedical Science - Category: Laboratory Medicine Tags: Br J Biomed Sci Source Type: research

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Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
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Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Authors: Huang T, Xu C, Xiao J, Wang Q, Wang Y, Zhang Y, Bai D, Zhou F, Zhao X Abstract Circulating tumor cells (CTCs) are widely used in cancer screening and monitoring. The present study focused on investigating the optimal time for the postoperative CTC detection in patients with colorectal cancer (CRC) to obtain more accurate results and facilitate subsequent treatment. By subtraction enrichment immunofluorescence in situ hybridization detection of CTCs, the present study demonstrated that different postoperative detection times in CRC substantially influenced the CTC numbers. In total, 134 subjects were enroll...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
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Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
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Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
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Source: Current Oncology - Category: Cancer & Oncology Tags: Curr Oncol Source Type: research
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Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
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Source: International Journal of Clinical and Experimental Pathology - Category: Pathology Authors: Tags: Int J Clin Exp Pathol Source Type: research
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Source: Annali Italiani di Chirurgia - Category: Surgery Tags: Ann Ital Chir Source Type: research
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