GSE140104 Retinoic acid exerts disease stage-dependent and tissue-specific effects on pristane-induced lupus

Contributors : Jiyoung Lee ; Song Li ; Leila Abdelhamid ; Xavier Cabana-Puig ; Brianna Swartwout ; Jiyoung Lee ; Song Li ; Sha Sun ; Yaqi Li ; A C Ross ; Thomas Cecere ; Tanya LeRoith ; Haifeng Wang ; Christopher M Reilly ; Xin M LuoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe previously showed that all-trans-retinoic acid (tRA), an active metabolite of vitamin A, exacerbated pre-existing autoimmunity in lupus; however, its effects before the development of autoimmunity are unknown. Here, using a pristane-induced model, we show that tRA exerts tissue-specific effects when given at the initiation vs. continuation phase of lupus. Unlike tRA treatment during active disease, pre-pristane treatment with tRA aggravated glomerulonephritis through increasing renal expression of pro-fibrotic protein laminin β1, activating bone marrow conventional dendritic cells (cDCs), and upregulating the interaction of ICAM-1 and LFA-1 in the spleen indicating an active process of leukocyte activation and trafficking. Transcriptomic analysis revealed that prior to lupus induction, tRA significantly upregulated the expression of genes associated with cDC activation and migration. Post-pristane tRA treatment, on the other hand, did not significantly alter the severity of glomerulonephritis; rather, it exerted immunosuppressive functions of decreasing circulatory and renal deposition of autoantibodies as well as suppressing the renal expressio...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research