Effect Simultaneous Delivery with P-Glycoprotein Inhibitor and Nanoparticle Administration of Doxorubicin on Cellular Uptake and In Vitro Anticancer Activity

In this study, we encapsulated the Ver and Dox in PLGA nanoparticles to inhibit the P-gp drug efflux in breast cancer. Moreover, the effect of either Dox solution (DoxS), Dox nanoparticles (DoxNP), DoxS+ VerS, DoxNP+VerS, DoxNP + VerNP or Dox-VerNP was evaluated. It was found that co administration of DoxNP with VerNP (70.76%) showed similar cellular uptake of Dox to Dox/Ver combination solution (70.62%). However it is observed that DoxNP+ VerNP has the highest apoptotic activity (early apoptotic 13.52±0.06%, late apoptotic 53.94±0.15%) on human breast adenocarcinoma (MCF 7) cells. Hence, it is suggested that DoxNP + VerNP is a promising administration for tumor therapy.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research