Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy- Δ 12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGF β -1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 μ g/ml and treated with 15d-PGJ2 1 and 2 μ g/ml, and ScLL 2 and 5 μ g/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGF β -1 release, by ELISA. Positive control was cells kept in Dulbecco ’ s Modified Eagle ’ s Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett ’ s test ( α = 0.05). No significant difference was found in viability among experimental groups at 1h (p> 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p> 0.05), whereas the other groups had lower levels than the positive control (p< 0.05). IL-6 release was statistically higher for ScLL 5 μ g/ml and 15d-PGJ2 2 µg/ml at 1h, compared with the other treated groups and positive control (p< 0.05). No significant differences were found among the groups at 3h (p> 0.05), except for ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p< 0.05). No significant difference was found among the groups for TGF β -1 release (p> 0.05). Results indicated that ScLL 5 μ g/ml did not i...
Source: Brazilian Oral Research - Category: Dentistry Source Type: research