miRNA-mRNA profiling reveals prognostic impact of SMC1A 4 expression in acute myeloid leukemia.
miRNA-mRNA profiling reveals prognostic impact of SMC1A 4 expression in acute myeloid leukemia.
Oncol Res. 2020 Feb 14;:
Authors: Gadewal N, Kumar R, Aher S, Gardane A, Gaur T, Varma AK, Khattry N, Hasan SK
Abstract
Acute myeloid leukemia (AML) with NPM1 mutation is a disease driving genetic alteration with good prognosis. Although it has been suggested that NPM1 mutation induces chemo-sensitivity in leukemic cells, the underlying cause for the better survival of NPM1 mutated patients is still not clear. Mutant NPM1 AML has a unique microRNA and their target gene (mRNA) signature compared to wildtype NPM1. Dynamic regulation of miRNA-mRNA has been reported to influence the prognostic outcome. In the present study, in-silico expression data of miRNA and mRNA in AML patients was retrieved from genome data commons and differentially expressed miRNA and mRNA among NPM1 mutated (n=21) and NPM1 wildtype (n=162) cases were identified to establish a dynamic association at molecular level. In-vitro experiments using high throughput RNA sequencing were performed on human AML cells carrying NPM1 mutated and wildtype allele. The comparison of in-vitro transcriptomics data with in-silico miRNA-mRNA expression network data revealed down regulation of SMC1A. On establishing miRNA-mRNA interactive pairs it has been observed that hsa-mir-215-5p (LogFC: 0.957; p=0.0189) is involved in the downregulation of SMC1A (LogFC: -0.481; p=0.0464) in NPM1 mutat...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research
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