Magnetic solid phase extraction followed with LC-MS/MS for determination of glimepiride in beagle dog plasma and its application to bioequivalence study
In this study, the core-shell polydopamine-coated magnetic nanomaterials were synthesized with one-step approach and applied as magnetic solid phase extraction adsorbents combined with LC-MS/MS for quantification of the diabetes drug glimepiride in beagle dog plasma. The Fe3O4@PDA nanomaterials have strong magnetic response, good dispersibility in aqueous solution, and numerous binding sites for π–π interaction and hydrogen bonding with glimepiride. Based on these merits, glimepiride could be quickly extracted and separated from plasma within 10min. The established method has good linearity (1-2000ng•mL-1), low quantification limit (1ng•mL-1), good precision (RSDs≤12%), and satisfactory extraction recovery (71.20-85.70%). Moreover, we successfully applied this method to the bioequivalence study of generic and innovator products of glimepiride in beagle dog plasma.
Publication date: Available online 6 April 2020Source: Indian Heart JournalAuthor(s): Samit Ghosal, Binayak Sinha, Jignesh Ved, Mansij Biswas
Purpose of review Hyperlipidemia, hypertension, diabetes and related metabolic disorders increase the risk for cardiovascular disease (CVD). Despite significant progress in the identification of key mechanisms and genetic polymorphisms linked to various CVDs, the rates of CVDs continue to escalate, underscoring the need to evaluate additional mechanisms for more effective therapies. Environment and lifestyle changes can alter epigenetic mechanisms mediated by histone modifications and long noncoding RNAs (lncRNAs) which play important roles in gene regulation. The review summarizes recent findings on the role of epigenet...
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Condition: Diabetic Foot Ulcer Interventions: Device: PulseFlow DF boot; Device: Usual standard of care; Device: Sham Sponsors: University of Leeds; The Leeds Teaching Hospitals NHS Trust Completed
Condition: Type1diabetes Intervention: Behavioral: 4T Education and Care Sponsors: Stanford University; National Institutes of Health (NIH); National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Not yet recruiting
Conditions: Non-Alcoholic Fatty Liver Disease; Diabetes Mellitus, Type 2 Intervention: Device: LiverMultiscan Sponsor: Perspectum Diagnostics Ltd Not yet recruiting
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