Proteomic characterization of lignocellulolytic enzymes secreted by the insect-associated fungus, Daldinia decipiens oita, isolated from the forest in northern Japan.

In this study, an insect-associated fungus, Daldinia decipiens oita was isolated as a potential symbiotic fungus of female Xiphydria albopicta captured from Hokkaido forest. This fungus was grown in seven different media containing a single carbon source, glucose, cellulose, xylan, mannan, pectin, poplar, or larch, and the secreted proteins were identified by LC-MS/MS. 128 CAZymes including domains of 92 glycoside hydrolases (GHs), 15 carbohydrate esterases (CEs), 5 polysaccharide lyases (PLs), 17 auxiliary activities (AAs), and 11 carbohydrate-binding modules (CBMs) were identified, and these are involved in degradation of cellulose and hemicellulose but not lignin. Together with the result of polysaccharide-degrading activity measurements, we concluded that D. decipiens oita tightly regulates the expression of these CAZymes in response to the tested plant cell wall materials. Overall, this study described the detailed proteomic approach of a woodwasp-associated fungus and revealed that the new isolate, D. decipiens oita, secretes diverse CAZymes to efficiently degrade lignocellulose in the symbiotic environment.ImportanceRecent studies show the potential impacts of insect symbiont microbes on biofuels application with regards to their degradation capability of a recalcitrant plant cell wall. In this study, we describe a novel fungal isolate, D. decipiens oita, as a single symbiotic fungus from the Xiphydria woodwasp found in northern forests of Japan. Our detailed secretome...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research