Lipoxin A4 reduces house dust mite and TNFα-induced hyperreactivity in the mouse trachea

Publication date: Available online 15 February 2020Source: Prostaglandins & Other Lipid MediatorsAuthor(s): Willem Abma, Malin Noreby, Craig E. Wheelock, Sven-Erik Dahlén, Mikael Adner, Jesper SäfholmAbstractLipoxin A4 (LXA4) is considered a specialised pro-resolving mediator that decreases inflammation: however, pro-inflammatory effects have been described in the airways. Here, we investigated whether LXA4 could influence airway hyperreactivity induced in mouse trachea by house dust mite extract (HDM) or TNFα. Intranasal instillation of HDM caused a serotonin (5-HT) mediated airway hyperreactivity ex vivo (Emax: 78.1 ± 16.2% versus control 12.8 ± 1.0%) that was reduced by LXA4 installation one hour prior to HDM (Emax: 49.9 ± 11.4%). Also, in isolated tracheal segments cultured for four days, HDM induced a hyperreactivity (Emax: 33.2 ± 3.1% versus control 9.0 ± 0.7%) that was decreased by LXA4 (Emax: 18.7 ± 1.5%). One part of the HDM-induced hyperreactivity could be inhibited by the TNFα-inhibitor etanercept. TNFα-induced upregulation of 5-HT responses (Emax: 51.3 ± 1.2% versus control 13.9 ± 0.5%) was decreased by 10-1000 nM LXA4. In precontracted tracheal segments, LXA4 had no relaxing effect. Overall, LXA4 was able to decrease airway hyperreactivity induced by both HDM and TNFα, thus having a sub-acute anti-inflammatory effect in airway inflammation.

Source: Prostaglandins and Other Lipid Mediators - February 16, 2020 Category: Lipidology Source Type: research