Whole-cell Fungal-mediated Structural Transformation of Anabolic Drug Metenolone Acetate into Potent Anti-inflammatory Metabolites

Publication date: Available online 15 February 2020Source: Journal of Advanced ResearchAuthor(s): Mahwish Siddiqui, Atia-tul-Wahab, Almas Jabeen, Yan Wang, Wei Wang, Atta-ur-Rahman, M. Iqbal ChoudharyAbstractSeven new derivatives, 6α-hydroxy-1-methyl-3-oxo-5α-androst-1-en-17-yl acetate (2), 6α,17β-dihydroxy-1-methyl-3-oxo-5α-androst-1-en (3), 7β-hydroxy-1-methyl-3-oxo-5α-androst-1-en-17-yl acetate (4), 15β,20-dihydroxy-1-methyl-3-oxo-5α-androst-1-en-17-yl acetate (5), 15β-hydroxy-1-methyl-3-oxo-5α-androst-1-en-17-yl acetate (6), 12β,17β-dihydroxy-1-methyl-3-oxoandrosta-1,4-dien (11), and 7β,15β,17β-trihydroxy-1-methyl-3-oxo-5α-androst-1-en (14), along with six known metabolites, 17β-hydroxy-1-methyl-3-oxoandrosta-1,4-dien (7), 17β-hydroxy-1-methyl-3-oxo-5α-androst-1-en (8), 17β-hydroxy-1-methyl-3-oxo-5β-androst-1-en (9), 1-methyl-5β-androst-1-en-3,17-dione (10), 1-methyl-3-oxoandrosta-1,4-dien-3,17-dione (12), and 17β-hydroxy-1α-methyl-5α-androstan-3-one (13) of metenolone acetate (1), were synthesized through whole-cell biocatalysis with Rhizopus stolonifer, Aspergillus alliaceous, Fusarium lini, and Cunninghamella elegans. Atamestane (12), an aromatase inhibitor, was synthesized for the first time via F. lini-mediated transformation of 1 as the major product. Hydroxylation, dehydrogenation, and reduction were occurred during biocatalys...
Source: Journal of Advanced Research - Category: Research Source Type: research

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Authors: Papachristou SG, Iosifidis E, Sipsas NV, Gamaletsou MN, Walsh TJ, Roilides E Abstract Introduction: Osteoarticular fungal infections (OAFIs) complicate the clinical course of high-risk patients, including immunosuppressed individuals. Their management, however, despite being intricate, is governed by evidence arising from sub-optimal quality research, such as case series. Guidelines are scarce and when present result in recommendations based on low quality evidence. Furthermore, the differences between the management of immunocompromised and immunocompetent patients are not distinct. This is a narrative re...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
AbstractThe present research was conducted to assess the role of aeromycoflora around world heritage sites of Taxila and its relation with biodeterioration of stone monuments. The aerial mycoflora of six archaeological sites was recorded for one year to monitor the seasonal variations and transportation of fungal spores. Thirty-two fungal species belonging to twenty genera were isolated throughout the whole sampling period. The fungal generaAlternaria, Aspergillus,Cladosporium,Fusarium,Mucor,Helminthosporium,Curvularia andPenicillium were prevalent, whereas at species levelAlternaria alternata was dominant followed byAsper...
Source: Aerobiologia - Category: Environmental Health Source Type: research
Publication date: Available online 26 March 2020Source: Journal de Mycologie MédicaleAuthor(s): Farideh Zaini, Ensieh Lotfali, Azam Fattahi, Ensieh Lotfali, Emmanuel Siddig, Shirin Farahyar, Ebrahim Kouhsari, Mojtaba Saffari
Source: Journal of Medical Mycology - Category: Biology Source Type: research
ConclusionOur results showed that there is no difference in the FEV1 and forced vital capacity betweenAspergillus positive and negative patients in any asthma severity group.
Source: The Clinical Respiratory Journal - Category: Respiratory Medicine Authors: Tags: ORIGINAL ARTICLE Source Type: research
Molecules, Vol. 25, Pages 1518: N-Pyrazinoyl Substituted Amino Acids as Potential Antimycobacterial Agents—The Synthesis and Biological Evaluation of Enantiomers Molecules doi: 10.3390/molecules25071518 Authors: Martin Juhás Lucie Kučerová Ondřej Horáček Ondřej Janďourek Vladimír Kubíček Klára Konečná Radim Kučera Pavel Bárta Jiří Janoušek Pavla Paterová Jiří Kuneš Martin Doležal Jan Zitko Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (Mtb), each year causi...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the highest toxicity and is labeled as a group I carcinogen in humans and animals. Consequently, the proper control of AFB1 produced from biofilms in food and feed has long been recognized. Moreover, many biosensors have been applied to monitor AFB1 in biofilms in food. Additionally, in recent years, novel molecula...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 25 March 2020Source: Medical Mycology Case ReportsAuthor(s): Chittaranjan Routray, Casmiar Nwaigwe
Source: Medical Mycology Case Reports - Category: Biology Source Type: research
ConclusionsThe findings reveal that propolis extract, have a significant inhibitory effect on important genes for aflatoxin biosynthesis pathway in aflatoxin production.
Source: Journal of Environmental Health Science and Engineering - Category: Environmental Health Source Type: research
AbstractPurpose of ReviewThis review summarizes current treatment options for echinocandin-resistantCandida spp. (ERC) and azole-resistantAspergillus fumigatus (ARAF), emphasizing recent in vitro/in vivo data, clinical reports, and consensus statements.Recent FindingsAdvances in ERC and ARAF treatment are limited to specific antifungal combinations and dose optimization but remain reliant on amphotericin products. Although novel antifungals may provide breakthroughs in the treatment of resistant fungi, these agents are not yet available. Early identification and appropriate treatment remain a paramount, albeit elusive, tas...
Source: Current Fungal Infection Reports - Category: Infectious Diseases Source Type: research
a Ioannou From the organic extracts of five bacterial strains isolated from marine sediments collected in the East Mediterranean Sea, three new (15, 16, 31) and twenty-nine previously reported (1–14, 17–30, 32) metabolites bearing the 2,5-diketopiperazine skeleton were isolated. The structures of the chlorinated compounds 15, 16, and 31 were elucidated by extensive analysis of their spectroscopic data (NMR, MS, UV, IR). Compounds 15 and 16 were evaluated for their antifungal activity against Candida albicans and Aspergillus niger but were proven inactive. The relevant literature is supplemented with...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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