De novo discovery of metabolic heterogeneity with immunophenotype-guided imaging mass spectrometry

ConclusionsWe developed the SPACiAL workflow to provide immunohistochemistry-guided in situ metabolomics on intact tissue sections. Diminishing the workload by automated recognition of histological and functional features, the pipeline allows comprehensive analyses of metabolic heterogeneity. The multimodality of immunohistochemical staining and extensive molecular information from imaging mass spectrometry has the advantage of increasing both the efficiency and precision for spatially resolved analyses of specific cell types. The SPACiAL method is a stepping stone for the objective analysis of high-throughput, multi-omics data from clinical research and practice, that is required for diagnostics, biomarker discovery or therapy response prediction.Graphical abstract
Source: Molecular Metabolism - Category: Endocrinology Source Type: research