Ketamine treatment affects hippocampal but not cortical mitochondrial function in prepubertal rats.

Ketamine treatment affects hippocampal but not cortical mitochondrial function in prepubertal rats. Int J Dev Neurosci. 2020 Feb 13;: Authors: Czerniczyniec A, Karadayian AG, Bustamante J, Lores-Arnaiz S Abstract Previous reports have shown that ketamine triggered apoptosis in immature developing brain involving mitochondrial mediated pathways. However, no data for ketamine effects on hippocampal and cortical mitochondrial function are available in pre-pubertal rats. Twenty-one days-old Sprague-Dawley rats received ketamine (40 mg/Kg i.p.) for 3 days and were sacrificed 24 hours after last injection. Hippocampal mitochondria from ketamine-treated rats showed decreased malate-glutamate state 4 and 3 respiratory rates and an inhibition in Complex I and IV activities. Hippocampal mitochondrial membrane depolarization and mitochondrial permeability transition induction were observed. This was not reflected in an increment of H2 O2 production probably due to increased Mn-SOD and catalase activities, 24 hours after treatment. Interestingly, increased H2 O2 production rates and cardiolipin oxidation were found in hippocampal mitochondria shortly after ketamine treatment (45 min). Unlike hippocampus, ketamine did not affect mitochondrial parameters in brain cortex, being this area less vulnerable to suffer ketamine-induced oxidative damage. Results provide evidences that exposure of pre-pubertal rats to ketamine leads to an induction of mito...
Source: International Journal of Developmental Neuroscience - Category: Neuroscience Authors: Tags: Int J Dev Neurosci Source Type: research