Policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedling extracts according to cultivar and growth time

Publication date: Available online 11 February 2020Source: Food ChemistryAuthor(s): Ji Eun Ra, So-Yeun Woo, Kwang-Sik Lee, Mi Ja Lee, Hyun Young Kim, Hyeon Mi Ham, Ill-Min Chung, Du Hyun Kim, Jin Hwan Lee, Woo Duck SeoAbstractPolicosanols is a health promoting aliphatic alcohol known as lipid-lowing agent.To enable maximising the functional properties of wheat, this research investigates the policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedlings according to cultivars and growth times. GC-MS revealed six policosanols that differed markedly in content between 17 cultivars, especially, octacosanol (8) showed the most predominant component (49-83%), varying significantly in average concentrations with growth times as 361.4 (3 days) → 613.0 (6 days) → 203.1 (9 days) → 196.5 (12 days) → 50.9 mg/100 g (19 days). The highest average policosanol (738.7 mg/100 g) exhibited after 6 days, while the lowest was 104.4 mg/100 g on 19 days. Moreover, the wheat cultivars including Shinmichal 1, Anbaek, Namhae, and Joah at 6 days may be recommended as potential sources because of high policosanols (921.7–990.6 mg/100 g). Western blot analysis revealed markedly higher AMPK activation in cells treated with the hexane extracts (150–370% at 100 μg/ml) and octacosanol (8) possessed potent AMPK activator (control; 100 → 280% at 200 μg/ml). It is confirmed that the AMPK activation by wheat seedlings are posi...
Source: Food Chemistry - Category: Food Science Source Type: research