Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein–ligand complex.
Source: Springer protocols feed by Imaging/Radiology - Category: Radiology Source Type: news