Liposome-based nanocarrier loaded with a new quinoxaline derivative for the treatment of cutaneous leishmaniasis

In this study, we developed hyaluronic acid (HA)-coated liposomes by different methods that can encapsulate a new quinoxaline derivative, the LSPN331, to increase its solubility and improve its bioavailability. The surface modification of liposomes and their physicochemical characteristics may depend on the coating method, which may be a critical parameter with regard to the route of administration of the antileishmanial drug. Liposomes with identical phospholipid composition containing the same drug were developed, and different biological responses were verified, and our hypothesis is that it is related to the type of modification of the surface. Different physicochemical characterization techniques (dynamic light scattering, transmission electron microscopy and UV–vis quantification of labeled-HA) were used to confirm the successful modification of liposomes as well as their stability upon storage. The encapsulation of LSPN331 was performed using HPLC method, and the entrapment efficiency (EE%) was satisfatory in all formulations, considering results of similar formulations in the literature. Furthermore, in vitro and in vivo studies were carried out to evaluate the efficacy against the parasite Leishmania amazonensis. The in vitro activity was maintained or even improved and HA-coated liposomes showed the ability to target to the site of action by the proposed routes of administration, topically and intravenously. Both formulations are promising for future tests of ...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research

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ConclusionsOur data indicates that dietary glutamine supplementation may act as a promising adjuvant for the treatment of visceral leishmaniasis.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
ConclusionOur results support the role ofPh.longicuspis,Ph.perfiliewi, andPh.perniciosus inL.infantum transmission. Furthermore, these species could be involved inL.major andL.killicki life cycles. The combination of the parasite detection and the blood meal analysis in this study highlights the incrimination of the identified vertebrate inLeishmania transmission. In addition, we quantify for the first time the parasite load in naturally infected sand flies caught in Tunisia. These findings are relevant for a better understanding ofL.infantum transmission cycle in the country. Further investigations and control measures ar...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
ConclusionsThese results demonstrate that the thymus of dogs can be parasitized byL. infantum, which may generate inflammatory reactions leading to alterations in thymic microarchitecture.
Source: Immunity, Inflammation and Disease - Category: Allergy & Immunology Authors: Tags: ORIGINAL RESEARCH Source Type: research
This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of theLeishmania donovani complex.
Source: eLife - Category: Biomedical Science Tags: Genetics and Genomics Microbiology and Infectious Disease Source Type: research
In conclusion, our findings indicate that these aptamers could be used for LiH3 histone detection and, in consequence, as potential biosensing molecules in a diagnostic tool for leishmaniasis. PMID: 32200890 [PubMed - in process]
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
(Funda ç ã o de Amparo à Pesquisa do Estado de S ã o Paulo) The study was conducted in Brazil and mapped the genes that are activated when mice resistant to Leishmania are infected. Molecules involved in the signaling pathway responsible for combating the parasite can serve as biomarkers for the evaluation of prognosis.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Abstract PIN1 proteins are a class of peptidyl prolyl cis-trans isomerases (PPIases), which have been implicated in numerous cellular functions like cell cycle progression, transcriptional control, signal transduction, promotion of oncogenesis and host-parasite interactions. In this work, the unfolding mechanism of a single domain PIN1 from Leishmania major (LmPIN1) has been characterized during thermal and denaturant-induced unfolding by differential scanning calorimetry (DSC), fluorescence and circular dichroism. Further, MD simulations have been performed to structurally probe the possible stages of its unfoldi...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
An outbreak of leishmaniosis was studied in cats and dogs housed together with no separation in an animal shelter in Israel.
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
by Myrthe Pareyn, Abena Kochora, Luca Van Rooy, Nigatu Eligo, Bram Vanden Broecke, Nigatu Girma, Behailu Merdekios, Teklu Wegayehu, Louis Maes, Guy Caljon, Bernt Lindtj ørn, Herwig Leirs, Fekadu Massebo BackgroundCutaneous leishmaniasis (CL) is a major public health concern in Ethiopia. However, knowledge about the complex zoonotic transmission cycle is limited, hampering implementation of control strategies. We explored the feeding behavior and activity of the vector (Phlebotomus pedifer) and studied the role of livestock in CL transmission in southwestern Ethiopia. MethodsBlood meal origins of engorged sand flies ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Conclusion: It was determined that none of the selected resistant L. tropica isolates showed resistance to Amphotericin B and that was also shown statistically (p
Source: Turkish Society for Parasitology - Category: Parasitology Authors: Tags: Turkiye Parazitol Derg Source Type: research
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